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. 2021 Jan 26;22(8):1385–1390. doi: 10.1002/cbic.202000679

Table 1.

Activity and stability of TtPyNP in aqueous solution.

T [°C]

k obs [s−1][a]

t 1/2 [b]

pTTN [106][c]

100

215.6

2.2 min

0.028

90

117.1

100.2 min

1.296

80

45.7

19.5 h

15.139

[a] Determined as initial rate with 1 a at pH 9 and the respective temperature as shown in Figure 1C. [b] Determined from incubation experiments from the incubation time‐dependent decay of enzymatic activity (initial rate) at pH 9 and the respective temperature (please see the externally hosted Supporting Information for all raw and calculated data). [20] The activity assay was performed at 60 °C to ensure enzyme stability during the assay. [c] Determined via multiplication of k obs and the mean catalyst lifetime τ as detailed in the Supporting Information.