Table 1.
Activity and stability of TtPyNP in aqueous solution.
T [°C] |
k obs [s−1][a] |
t 1/2 [b] |
pTTN [106][c] |
---|---|---|---|
100 |
215.6 |
2.2 min |
0.028 |
90 |
117.1 |
100.2 min |
1.296 |
80 |
45.7 |
19.5 h |
15.139 |
[a] Determined as initial rate with 1 a at pH 9 and the respective temperature as shown in Figure 1C. [b] Determined from incubation experiments from the incubation time‐dependent decay of enzymatic activity (initial rate) at pH 9 and the respective temperature (please see the externally hosted Supporting Information for all raw and calculated data). [20] The activity assay was performed at 60 °C to ensure enzyme stability during the assay. [c] Determined via multiplication of k obs and the mean catalyst lifetime as detailed in the Supporting Information.