Table 2.

Phosphorolysis of 5‐substituted pyrimidine nucleosides by TtPyNP.^[a]

[a] Reactions were performed with 1 mM nucleoside (1 a–1 i), 50 mM potassium phosphate and 0.25 μg mL⁻¹ TtPyNP in 50 mM glycine/NaOH buffer at pH 9 and 80 °C. Data shown represent the average of two experiments. Standard deviations were all 5 % or below and were omitted for clarity. Please see the Supporting Information for more experimental details and the externally hosted Supporting Information for raw data and calculations. ^[20]