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. 2021 Feb 1;9(3):944–955. doi: 10.1111/andr.12965

Figure 3.

Figure 3

Effects of HBs exposure on Bcl2, Bax, and Apaf‐1 activation in sperm cells. The sperm sample were randomly divided into 4 groups: A, B, C, and N. Each group was divided into 4 subgroups, among which subgroups A2‐4, B2‐4, and C2‐4 were exposed to HBs at the final concentrations of 25, 50, and 100 μg/mL, respectively, as the test groups; subgroups A1, B1, and C1 were not exposed to HBs and were established as the blank control groups. Groups A, B, and C were incubated first with specific rabbit monoclonal antibodies against Bcl2, Bax, and Apaf‐1 (the primary antibodies), respectively, and then incubated with FITC‐labeled goat anti‐rabbit IgG (the secondary antibody). The four subgroups of group N (N1‐4) were exposed to HBs at final concentration of 0, 25, 50, and 100 μg/mL, respectively, and incubated only with the secondary antibody as the negative control.