Table 2.

Effect of HBs exposure on the transcription levels of Bcl2, Bax, and Apaf‐1 genes in sperm cells

HBs‐treated groups (µg/mL)	Bcl2		Bax		Apaf−1
	2−△△CT	FCV	2−△△CT	FCV	2−△△CT	FCV
0	1.00		1.00		1.00
25	0.29*	−3.45	1.28	1.28	1.26	1.26
50	0.18*	−5.56	1.52*	1.52	2.00*	2.00
100	0.10*	−10.00	1.80*	1.80	2.68*	2.68

Total RNA was extracted from sperm cells in the HBs (0, 25, 50, and 100 µg/mL)‐treated groups and subjected to RT‐qPCR to detect the transcription levels of the Bcl2, Bax, and Apaf‐1 genes, The data are presented as a fold change values (FCVs) for the transcription levels of the Bcl2, Bax, and Apaf‐1 genes in the test groups normalized to the transcription level of the internal control gene (GAPDH) and relative to those in the control group. Individual data were converted to a linear form using 2^−Ct method ²³ and were then subjected to ANOVA to determine whether the average transcription levels of the Bcl2, Bax, and Apaf‐1 genes differed significantly between the test and control groups.

^* P < 0.05.