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. 2021 Feb 18;31(5):522–539. doi: 10.1002/hipo.23314

TABLE 2.

Quantification of MC axons

Dorsal injection
Inner GFP (% of ML) Outer GFP (% of ML) Total width (% of ML)
D/V Hemisphere Upper Crest Lower Upper Crest Lower Upper Crest Lower
Dorsal sections Ipsilateral 7.02 ± 1.09% 4.59 ± 0.63% 3.46 ± 0.44% 65.69 ± 1.16% 63.61 ± 1.45% 64.12 ± 1.43% 58.66 ± 0.97% 59.02 ± 1.56% 60.66 ± 1.57%
Contralateral 7.65 ± 0.95% 6.53 ± 1.04% 2.91 ± 0.57% 64.71 ± 1.22% 64.32 ± 0.99% 64.08 ± 1.23% 57.06 ± 1.04% 57.78 ± 0.92% 61.34 ± 1.60%
Ventral sections Ipsilateral 41.17 ± 2.28% 37.00 ± 1.66% 37.44 ± 1.53% 68.04 ± 1.78% 59.35 ± 2.10% 61.11 ± 1.87% 26.87 ± 1.64% 22.35 ± 1.31% 23.67 ± 1.46%
Contralateral 38.74 ± 1.15% 38.10 ± 0.87% 34.73 ± 2.10% 65.12 ± 0.84% 61.69 ± 1.13% 62.88 ± 1.62% 26.39 ± 1.24% 23.41 ± 1.27% 27.86 ± 2.12%
Ventral injection
Inner GFP (% of ML) Outer GFP (% of ML) Total width (% of ML)
D/V Hemisphere Upper Crest Lower Upper Crest Lower Upper Crest Lower
Dorsal sections Ipsilateral 1.12 ± 0.39% 0.74 ± 0.25% 0.81 ± 0.32% 22.69 ± 0.95% 25.28 ± 0.84% 21.37 ± 1.10% 21.40 ± 1.04% 24.29 ± 1.00% 20.41 ± 0.96%
Contralateral 1.83 ± 0.41% 0.44 ± 0.07% 0.37 ± 0.13% 23.33 ± 0.72% 23.94 ± 0.77% 21.27 ± 1.01% 21.40 ± 0.64% 23.20 ± 0.77% 20.54 ± 0.97%
Ventral sections Ipsilateral 0.61 ± 0.19% 0.47 ± 0.17% 0.31 ± 0.02% 25.44 ± 0.79% 23.96 ± 0.60% 25.55 ± 1.40% 24.64 ± 0.84% 23.21 ± 0.57% 24.96 ± 1.38%
Contralateral 2.17 ± 0.47% 0.66 ± 0.17% 0.49 ± 0.12% 23.91 ± 0.78% 23.31 ± 0.65% 22.59 ± 0.86% 21.65 ± 0.53% 22.45 ± 0.63% 21.94 ± 0.88%

Note: Quantification of the GFP+ axon in mice that were injected in the (1) dorsal or (2) ventral DG with AAV‐EF1A‐DIO‐eYFP. Values are presented as a percent of the total molecular layer (ML) width in the upper, crest, or lower blade of the DG. For each analysis, the measurements are separated by dorsal versus ventral location of the sections that were used, and the injected (ipsilateral) versus the noninjected (contralateral) hemisphere. D/V, dorsal/ventral.