TABLE 2.
Dorsal injection | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Inner GFP (% of ML) | Outer GFP (% of ML) | Total width (% of ML) | ||||||||
D/V | Hemisphere | Upper | Crest | Lower | Upper | Crest | Lower | Upper | Crest | Lower |
Dorsal sections | Ipsilateral | 7.02 ± 1.09% | 4.59 ± 0.63% | 3.46 ± 0.44% | 65.69 ± 1.16% | 63.61 ± 1.45% | 64.12 ± 1.43% | 58.66 ± 0.97% | 59.02 ± 1.56% | 60.66 ± 1.57% |
Contralateral | 7.65 ± 0.95% | 6.53 ± 1.04% | 2.91 ± 0.57% | 64.71 ± 1.22% | 64.32 ± 0.99% | 64.08 ± 1.23% | 57.06 ± 1.04% | 57.78 ± 0.92% | 61.34 ± 1.60% | |
Ventral sections | Ipsilateral | 41.17 ± 2.28% | 37.00 ± 1.66% | 37.44 ± 1.53% | 68.04 ± 1.78% | 59.35 ± 2.10% | 61.11 ± 1.87% | 26.87 ± 1.64% | 22.35 ± 1.31% | 23.67 ± 1.46% |
Contralateral | 38.74 ± 1.15% | 38.10 ± 0.87% | 34.73 ± 2.10% | 65.12 ± 0.84% | 61.69 ± 1.13% | 62.88 ± 1.62% | 26.39 ± 1.24% | 23.41 ± 1.27% | 27.86 ± 2.12% |
Ventral injection | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Inner GFP (% of ML) | Outer GFP (% of ML) | Total width (% of ML) | ||||||||
D/V | Hemisphere | Upper | Crest | Lower | Upper | Crest | Lower | Upper | Crest | Lower |
Dorsal sections | Ipsilateral | 1.12 ± 0.39% | 0.74 ± 0.25% | 0.81 ± 0.32% | 22.69 ± 0.95% | 25.28 ± 0.84% | 21.37 ± 1.10% | 21.40 ± 1.04% | 24.29 ± 1.00% | 20.41 ± 0.96% |
Contralateral | 1.83 ± 0.41% | 0.44 ± 0.07% | 0.37 ± 0.13% | 23.33 ± 0.72% | 23.94 ± 0.77% | 21.27 ± 1.01% | 21.40 ± 0.64% | 23.20 ± 0.77% | 20.54 ± 0.97% | |
Ventral sections | Ipsilateral | 0.61 ± 0.19% | 0.47 ± 0.17% | 0.31 ± 0.02% | 25.44 ± 0.79% | 23.96 ± 0.60% | 25.55 ± 1.40% | 24.64 ± 0.84% | 23.21 ± 0.57% | 24.96 ± 1.38% |
Contralateral | 2.17 ± 0.47% | 0.66 ± 0.17% | 0.49 ± 0.12% | 23.91 ± 0.78% | 23.31 ± 0.65% | 22.59 ± 0.86% | 21.65 ± 0.53% | 22.45 ± 0.63% | 21.94 ± 0.88% |
Note: Quantification of the GFP+ axon in mice that were injected in the (1) dorsal or (2) ventral DG with AAV‐EF1A‐DIO‐eYFP. Values are presented as a percent of the total molecular layer (ML) width in the upper, crest, or lower blade of the DG. For each analysis, the measurements are separated by dorsal versus ventral location of the sections that were used, and the injected (ipsilateral) versus the noninjected (contralateral) hemisphere. D/V, dorsal/ventral.