Figure 4.

Phenotypic assessment of compound efficiency on plasmodial FNT inhibition in yeast. A) Representative 96‐well‐plate used for the phenotypic screening of FNT inhibitors and their efficiency on the FNTs from the five human‐pathogenic Plasmodium species (Pf: P. falciparum, Pv: P. vivax, Pm: P. malariae, Po: P. ovale, Pk: P. knowlesi). A blue color of the resazurin viability marker indicates decreased yeast viability due to FNT inhibition. Cell viability of PfFNT‐expressing yeast grown on l‐lactate medium in the presence of increasing concentrations of B) MMV007839, C) BH297, and D) BH267.meta was determined by using resazurin. E) Heatmap visualizing compound efficacy on FNT inhibition. The color code indicates the threshold concentration at which a 50 % decrease in yeast viability was reached.