FIGURE 1.

Universal PCR primers for loxP and FRT sites. (a) Design of loxP‐specific primer with modification at 5’ end containing BamHI site. (b) Design of FRT‐specific primer with modification at 5’ end containing BamHI site. (c) How to use loxP‐ and FRT‐specific primers for validation of conditional alleles. Green: palindrome right and left arms of loxP and FRT. Blue: spacer sequence to ensure the amplified fragment contains intact restriction site. Yellow: restriction endonuclease recognition site. Red nucleotide in (a) and (b): non complementary sequence. Red bar in (c): primer sequence complementary to the core sequence of loxP or FRT