FIGURE 2.

PCR analyses of conditional floxed and Flrted alleles. (a) The allele structure of the conditional floxed and Flrted knockout mouse C57BL/6N‐Gal^{tm1a(KOMP)Wtsi}/G08 (RBRC05903), posted on the IMPC website (https://www.mousephenotype.org/). Three floxed (red arrows) and two Flrted (green arrow) intervals expected as PCR products by loxP‐ and FRT‐PCR tests were estimated in silico. (b) Results of loxP‐ and FRT‐tests using primers with 5' end containing four different restriction sites and control. M: 1 kb Plus DNA Ladder (0.1–10.0 kb; New England BioLabs Japan). S: Genomic DNA of Gal^{tm1a /+} heterozygous mice. B6: Genomic DNA of wild‐type B6 control