Figure 7.

Effects of diperodon on SMYD3 in HCT116 colon cancer cells. A) Co‐immunoprecipitates stained with anti‐SMYD3 and anti‐HSP90 after treatment with 50 μM of diperodon in the form of a racemic mixture, isolated enantiomers or reconstituted racemic mixture. IgG was used as negative control. B) Chromatin fractions stained with anti‐SMYD3 and anti‐H3. HCT116 cells were cultured in serum deprivation and treated for 24 h with 50 μM of diperodon in the form of a racemic mixture, isolated enantiomers or reconstituted racemic mixture, 1 μM of 17‐AAG (Sigma), 100 μM of BCI‐121. SMYD3 recruitment on chromatin was enhanced by serum stimulation for 4 h with regular 10 % FBS cell medium. Uncropped blots are shown in Figure S10.