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Bioorthogonal chemistry combinations for dual functionalization of proteins (a) CuAAC + CuAAC. [79] (b) CuAAC + SPAAC. [50] (c) CuAAC + iEDDA. [25] (d) SPAAC + iEDDA. [80] (e) CuAAC + Oxime ligation. [81] (f) CuAAC+ iEDDA. [82] (Solid line refers to two moieties reacting with each other; dashed line refers to their orthogonal reactivities).
