Table 2.
Commonly used peptide tags and their respective enzymes.
|
Tag sequence[a] |
Enzyme |
|---|---|
|
GLNDIFEAQKIEWHE [108] |
Biotin ligase [108] |
|
LPXTGG [109] |
Sortase A [109] |
|
NHV [110] |
Butelase A [110] |
|
CaaX[b][111] |
Farmesyl transferase [111] |
|
LLQG [112] |
Microbial transglutaminase [112] |
|
GFEIDKVWYDLDA [113] |
Lipoate acid ligase A [113] |
|
CXPXR [114] |
Formylglycine Generating Enzyme [114] |
|
GDSLSWLLRLLN [115] |
Phosphopantetheinyl transferase [115] |
|
VDSVEGEGEEEGEE [116] |
Tubulin tyrosine ligase [116] |
|
AHIVMVDAYKPTK(Spytag) [117] |
SpyCatcher [117] |
[a] The capital letters are the abbreviation of different amino acids. “X” represents any amino acids. [b] “a” represents small aliphatic amino acids and “X” denotes one of Ala, Ser, Met or Glu residues amino acids in CaaX motif.