Figure 4.

Icaritin inhibits IKK complex formation. (A) Biochemical assay of IKK‐α in the presence of Icaritin or staurosporine. Values are mean ± SEM. (B) THP‐1 cells were pre‐treated with 20 μM Icaritin overnight, followed by 50 ng/mL TNFα treatment for 0.5 hr. After 300 ug protein pull‐down by the indicated antibody, the levels of IKK‐α and IKK‐β were examined by western blotting, 10% input serves as loading control. (C) THP‐1 or SMMC‐7721 cells were pretreated with indicated concentration of Icaritin overnight, followed by 50 ng/mL TNF‐α treatment for 0.5 h. The levels of p‐IKKα/β, IKK‐α, p‐IκBα, and IκB‐α were examined by western blotting, GAPDH was used as the loading control. Data are from a single experiment representative of three independent experiments.