Figure 2.

HAMLET induces maturation of Mo‐DCs. Flow cytometric analyses of primary human Mo‐DCs treated in SFM with HAMLET (red), native αLA (blue) or LPS (green) for 1‐h and grown overnight in fresh differentiation medium. (A) Gating strategy and dot plots of CD14, CD83 and HLA‐DR from cells treated with 0.1 mg/ml HAMLET or αLA or 100 ng/ml LPS. Gated on all viable cells, with percentage (%) in gate indicated. (B) Histograms represent mean fluorescence intensity (MFI; values in graphs) of indicated receptors, gated on all CD14^low/– Mo‐DCs (red box in A). (C) Individual data points of percentages (%) CD14^low/–CD83⁺ mature Mo‐DCs, with mean and SEM shown. Data from 4–8 individual experiments (n = 4‐8 donors). Statistics by Kruskal‐Wallis with Dunn's multiple comparisons test to untreated control. *P<0.05 and **P<0.01.