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. 2021 Feb 10;51(4):965–977. doi: 10.1002/eji.202048813

Figure 5.

Figure 5

The HAMLET‐induced phenotypes are mediated by calcium, NFκB and p38 MAPK signaling. Primary human Mo‐DCs (A) or Mo‐M (B) were pre‐treated with indicated inhibitor for 2‐h, stimulated with 0.1 mg/ml HAMLET (HL; red) or 100 ng/ml LPS (green) for 1‐h and subsequently grown overnight in fresh differentiation medium. The cells were analyzed by flow cytometry. Individual data points of relative % CD14low/– CD83+ Mo‐DC or CD14+CD86++ M1‐like Mo‐M to respective control (HL or LPS without inhibitor, but with PBS, ethanol or DMSO), with mean and SEM shown. Data from 8–12 individual experiments (n = 8‐12 donors). (C) Supernatants from Mo‐DCs (left) and Mo‐M (right) from A‐B were analyzed by CBA. Individual data points of relative cytokine concentration to respective control (HL or LPS without inhibitor, but with PBS, ethanol or DMSO), with mean and SEM shown. Data from 3 individual experiments (n = 3 donors). All statistics by paired t‐test to respective control. ns = not significant, *P<0.05, **P<0.01 and ***P<0.001.