Table 3.
Frequency of circulating immune cell subsets in children with asthma and age-matched non-asthmatic group.
| Cell Subsets | Asthma (n=17) | Healthy controls (n=11) | P-value* |
|---|---|---|---|
| Innate lymphoid cells1 | |||
| ILC1 | 51.09 (39.04, 100.62) | 21.91 (13.04, 67.60) | 0.022 |
| ILC2 | 151.8 (81.16, 335.17) | 8.40 (6.52, 11.46) | <0.001 |
| ILC3 NCR- | 25 (2.52, 70.99) | 0 (0, 0.53) | <0.001 |
| ILC3 NCR+ | 0 (0, 0.69) | 0 (0, 0) | 0.052 |
| Granulocytes | |||
| Eosinophils | 111914.8 ± 142033.3 | 63698.76 ± 34578.89 | 0.141 |
| Neutrophils | 280270.5 ± 137243 | 345993 ± 169330.6 | 0.865 |
| Dendritic cells | |||
| BDCA-1 | 30476.19 (16366.61, 37001.29) | 6503.078 (1912.066, 43572.04) | 0.111 |
| BDCA-3 | 4444.444 (2646.28, 21046.3) | 14217.13 (1572.327, 73949.58) | 0.706 |
| pDCs | 26904.76 (19498.61, 57909.61) | 16758.32 (3783.01, 40094.34) | 0.352 |
| NK cells | 41905.82 (33153.01, 68353.91) | 38138.79 (13537.55,56780.51) | 0.543 |
| B cells | 432004.3 (412312.2, 634458.6) | 287007.8 (234278.1, 486687) | 0.068 |
| T cells | |||
| CD4+ T cells | 528823.5 (517112.3, 591268.7) | 562661.8 (502188.2, 591235.2) | 0.724 |
| Activated CD4+ | 34910.71 (27368.62, 46767.48) | 34416.15 (25020.48, 79951.78) | 0.795 |
| Treg cells | 36234.1 (34276.74, 39402.04) | 32471.75 (27356.96, 40535.74) | 0.249 |
| CD8+ T cells | 189591.7 ± 140576.2 | 206728.8 ± 104836.4 | 0.732 |
| Activated CD8+ | 674.48 (247.83, 1730.77) | 1056.32 (684.84, 4197.76) | 0.094 |
| TCR-beta T cells | 742566.8 (683641.6, 832381) | 768274.4 (729063.5, 838161.9) | 0.759 |
| γδ-T cells | 81940.52 (58436.9, 115443.6) | 60867.0 (48278.07, 89825.17) | 0.249 |
Data are presented as mean ± SD or median (interquartile range). 1Innate lymphoid cells and granulocytes are per 106 CD45+ cells. Dendritic cells are per 106 human leukocyte antigen+ cells. B cells, T cells and NK cells adjusted are per 106 CD3+ cells. *Difference between groups analysed by Wilcoxon Rank Sum test (non-parametric data) or two-sample t-test (parametric data). P < 0.05 considered statistically significant. ILCs, innate lymphoid cells; NCR, natural cytotoxicity receptor; BDC, blood dendritic cells; PDC plasmacytoid dendritic cells; NK cells, natural killer cells; TCR-β, T cell receptor-β; Treg cells, T regulatory cells. Bold values indicate statistically significant difference (P<0.05) noted between the two groups.