FIGURE 2.

Gene expression changes in mouse corpus callosum after 4-week cerebral hypoperfusion. (A) Diagram for corpus callosum preparation. Four weeks after sham or BCAS operation, mice were sacrificed, and the corpus callosum samples were prepared. From each group, three mice were used for the RNAseq studies. CC: corpus callosum. (B) Gene expression levels of oligodendrocyte markers (Mbp and Mobp) were much higher than that of cortical neuron markers (Reln for layer I, Rasgrf2 for layer II/III, Pou3f2 for layers II-V, and Foxp2 for layer IV). (C) The principal component analysis (PCA) plot. (D) The MA plot. Blue dots represent the genes with adjusted p-value < 0.1 against the sham group. (E) The volcano plot. The red dots represent the genes that showed | log2 fold change| > 0.3 with adjusted p-value < 0.1 against the sham group. Please see Supplementary Table 2 for the list of differentially expressed genes between sham- and BCAS-operated mice.