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. 2021 Jun 17;9:695041. doi: 10.3389/fcell.2021.695041

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Copyright © 2021 Li, Huang, Zhang, Lu, Wei, Yang and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inositol-requiring enzyme-1 inhibits mTOR and reduces ER stress-apoptosis. Cells were cultured with 40 μM Bz-ATP, 1 μM 4μ8C, and 5 μM rapamycin. Western blotting (A,B) and qRT-PCR (C) were used to detect the protein and mRNA expression levels of IRE1, mTOR, PERK, caspase-12, CHOP, LC3B, and Beclin-1. Flow cytometry was used to detect the percentage of double-stained Annexin-V/PI cells (D) and the level of intracellular oxidative stress, ROS (E). (F) The caspase-3 activity assay; the statistical results are homogenized. Data are expressed as means ± standard deviations of at least three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01.