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. 2021 Jun 17;15:649485. doi: 10.3389/fnins.2021.649485

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Copyright © 2021 Trifunovic, Stevanovic, Milosevic, Ristic, Janjic, Bjelobaba, Savic, Bozic, Jakovljevic, Tesovic, Laketa and Lavrnja.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Timeline of the experimental design. Rats were immunized with the homogenate of the spinal cord and CFA. The rats were sacrificed at the onset of the first symptom (Eo), peak of disease (Ep), and end of symptoms (Ee). (B) Time course of experimental autoimmune encephalomyelitis (EAE) symptoms and variation in the weight of the animals. Data have been expressed as mean ± SEM of daily measurements of each animal. (C) Inflammation was observed using Hoechst stain (blue), while demyelination was detected using the MBP antibody (green fluorescence).