FIGURE 5.

Nitrosative/oxidative status assessment in the pituitary gland during EAE. Total nitrites (NOx; μmol/mg protein) were determined to assess nitrosative status. For oxidative status assessment, O₂^– (μmol/min/mg protein), GSH (μmol/mg protein), and MDA (nmol/mg protein) levels and enzymatic activities of GPx (M NADPH/mg protein), GSR (μmol NADPH/mg protein), catalase (U/mg protein), and SOD2 (U/mg protein) were determined in the pituitary gland at the onset (Eo), peak (Ep), and end (Ee) of EAE rats. Values are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 statistically significant difference compared with the control (C) group.