Figure 9.
Investigation of distinct human neuropeptide Y receptor 2 (hY2R) subpopulations by pulse‐chase labeling. Cys‐E3‐tagged hY2R were pulse labeled (red) by peptide‐templated NCL prior to agonist‐induced internalization. After the first stimulation, the remaining Cys‐E3‐hY2R, residing in the cell membrane, were chase labeled (light green) by peptide‐templated NCL. Chase‐labeled receptors were internalized upon agonist addition and co‐localized with chase‐labeled hY2R populations after >10 min (fused vesicles indicated by white arrows). Fusion of pulse‐ and chase‐labeled vesicles shown by (merged) yellow structures in the overlay pictures. Data adapted from Lotze, et al. [136]