TABLE 3.
Characteristics of JCPyV‐Specific T Cell Products Used in the Cohort
| Pt | PBMC Source | IFNγ Production, Spots/105 Cells | Cytotoxic activity, % Lysis at 2.5:1 E:T Ratio | Phenotype, % Positive Cells | |||||
|---|---|---|---|---|---|---|---|---|---|
| JCPyV VP1 + LT Peptides | Allogeneic PBMCs | JCPyV VP1 + LT Peptides | Allogeneic PHA Blasts | CD3+ | CD4+ | CD8+ | CD3−/CD56+ | ||
| 1 | Allogeneic (related third party) | 560 | 14 | 18 | 0 | 95 | 89 | 7 | 1 |
| 2 | Autologous | 900 | — | 41 | — | 93 | 56 | 35 | 6 |
| 3 | Autologous | 27 | — | 3 | — | 38 | 24 | 12 | 57 |
| 4 | Allogeneic (unrelated third party) | 43 | 13 | 35 | 8 | 97 | 84 | 13 | 2 |
| 5 | Autologous | 35 | — | 31 | — | 90 | 39 | 52 | 5 |
| 6 | Allogeneic (related third party) | 202 | nd | 43 | nd | 99 | 73 | 21 | 1 |
| 7 | Allogeneic (unrelated third party) | 123 | 8 | 37 | 14 | 82 | 71 | 8 | 15 |
| 8 | Autologous | 73 | — | 29 | — | 88 | 2 | 80 | 19 |
| 9 | Allogeneic (HSCT donor) | 63 | 3 | 10 | 0 | 95 | 85 | 8 | 2 |
E:T ratio = effector‐to‐target ratio; HSCT = hematopoietic stem cell transplantation; JCPyV = polyomavirus JC; IFNγ = interferon gamma; LT = large T protein; nd = not done; PBMCs = peripheral blood mononuclear cells; PHA = phytohemagglutinin; VP1 = viral capsid 1 protein.