Fig. 6.

Anthocyanin biosynthesis genes are downregulated in incolorata I ¹ petals of A. majus. (a) Wild‐type (WT) and delila mutant flowers were separated into tube and lobes for analysis of CHS, CHI, F3H, DFR, ANS and 3GT transcript abundance by qRT‐PCR in tube (T) and lobe (L) tissue of WT and delila mutant petals. (b) Petals from whole flowers of WT, ros^dorsea and inc I¹ : del were analysed for CHS, CHI, F3H, DFR, ANS and 3GT transcript levels by qRT‐PCR. Bar colour indicates pigmentation status. Means ± SEM, n = 3 biological replicates. Means that are significantly different (Student’s t‐test) from WT are indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001).