Fig. 9.

Bicolour patterning in the delila mutant arises from mis‐regulation of Incolorata I in flowers of A. majus. (a) Wild‐type (WT) and delila mutant flowers were analysed for Rosea1, WDR1, Delila, Del‐like and Incolorata I transcript levels by qRT‐PCR in tube (T) and lobe (L) tissue of WT and delila mutant petals. (b) Petals were analysed for Rosea 1, WDR1, Delila, Del‐like and Incolorata I transcript levels by qRT‐PCR. Bar colour indicates pigmentation status. Means ± SEM, n = 3 independent, biological replicates. Means that are significantly different (Student’s t‐test) from WT are indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (c) Model illustrating how bicolour patterning may result from the tube‐ and lobe‐centred activities of Del and Del‐like respectively regulating expression of Inc I and WDR1 which, in turn, is necessary for full Inc I activity in the MBW complex.