Figure 3.

Knockdown of MUC2, GALNT7 or FUT2 decreased the adhesion of C. butyricum to HT-29 cells. n=3. (A) Knockdown efficiencies of MUC2, GALNT7 and FUT2 were examined by RT-qPCR in HT29 cells, and then western blot analysis was performed to verify the knockdown efficiencies. (B) After knockdown of MUC2, GALNT7 or FUT2, HT-29 cells were stained with FITC labeled agglutinin. The mean fluorescence intensity of each picture is 33.44, 31.25, 7.19, 5.76, 4.91 arbitrary units (AU) using ImageJ software. (C) Adhesion of C. butyricum to HT-29 cells was assayed by measure of the fluorescence intensity with the microplate reader at 528 nm. The si-MUC2-2, si-GALNT7-2 and si-FUT2-1 were used in this adhesion experiment. Results are expressed as means and standard deviation of three independent experiments with **P < 0.01 and *P < 0.05.