Fig 3. Proteins dysregulated in maca^null/null testes including downregulated Kl-2, Kl-3, and Kl-5 revealed by mass spec.

Heatmap of protein levels that were significantly dysregulated in the maca^null/null testes compared with the testes from maca^+/+, maca^null/+, and maca^null/null with maca-EGFP rescue transgene determined by mass spec with TMT labeling. Log₂(fold-change compared with the means of maca^null/+) are shown. Four biological replicates for each genotype. Two proteins (at the top) were significantly upregulated (relative abundance >1.3, adjusted P-value <0.05) and 33 proteins were significantly downregulated (relative abundance <0.77, adjusted P-value <0.05) in maca^null/null compared with all the other three genotypes. Male fertility factor Kl-2 was most strongly and significantly downregulated (relative abundance 0.12 and adjusted P-value 1.7 x 10⁻⁵ in maca^null/null compared with maca^null/+). Proteins are sorted based on the chromosomal positions of the corresponding genes.