Fig 4. Poly-A+ RNA-seq analysis revealed RNAs dysregulated in maca^null/null testes including downregulated kl-2, kl-3, and kl-5 mRNAs.

(A, B) Heatmaps of RNA levels that are significantly upregulated or downregulated in the maca^null/null testes compared with the testes from maca^+/+, maca^null/+, maca^null/+ with maca-EGFP rescue transgene, and maca^null/null with maca-EGFP rescue transgene, determined by poly-A+ RNA-seq. Log₂(fold-change compared with the means of maca^null/+) are shown. Three biological replicates for each genotype. (A) Fifty genes were significantly upregulated (relative abundance >1.32, adjusted P-value <0.01) and (B) 38 genes were significantly downregulated (relative abundance <0.76, adjusted P-value <0.01) in maca^null/null compared with all the other four genotypes. Male fertility factor kl-2 mRNA was most strongly and significantly downregulated (relative abundance 0.10 and adjusted P-value 1.65 x 10⁻¹⁹⁶ in maca^null/null compared with maca^null/+). Genes are sorted based on their chromosomal positions.