Fig 10. RNA FISH for kl-3 transcripts shows exon 13 skipping in maca^null/null testes.

RNA FISH to visualize kl-3 transcripts in (A, B) maca^null/+ and (C, D) maca^null/null testes. Introns (Y-loop B, Alexa488-(AATAT)₆, green), exon 13 (blue), exon 14 (red), and DAPI (white). (A, C) Apical regions of testes including the spermatocyte growth region are shown. The apical tip of testis is marked by *. Scale bars are 50 μm. (B, D) Single spermatocyte nuclei (white dashed line) at each stage of kl-3 expression. Cytoplasmic mRNA granules are indicated by cyan arrows for maca^null/+, which contains signal from both exon 13 and exon 14, and by yellow arrows for maca^null/null, which contains signal from exon 14 but not exon 13. Scale bars are 5 μm.