Fig 11. RT-qPCR of kl-5 transcripts shows their reduction in maca^null/null testes.

Quantification of kl-5 transcripts in testis RNA by RT-qPCR. The RT step was performed with random hexamer primers. qPCR amplicons targeted by designed primer sets are indicated by orange bars. The primers span exon-exon junctions to amplify only spliced transcripts. Black lines indicate intron regions not included in the amplicons. Data were normalized to actin5C mRNA and the mean of maca^null/+. Mean +/- SD (n = 3 biological replicates). P-values <0.05 (Student’s t-test, unpaired, two-tailed) are indicated by *.