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. 2021 Jun 15;12:661811. doi: 10.3389/fimmu.2021.661811

Figure 2.

Figure 2

Assay of inflammasomes activation in Airway Macrophages (AMs). (A) AMs treated for 5 hours with LPS (B) Activation of NLRP3 upon priming with LPS for 2 hours and ATP for 30minutes. Cells were also treated in presence of the Selective NLRP3 inhibitor MCC950 to show specificity of the NLRP3 activation, (C) AMs cultured for 2 hours in serum and treated for 30 minutes with ATP without priming with LPS (D) AIM2 inflammasome activation: 1 hour of LPS priming followed by 4 hours dsDNA transfection, (E) NLRC4 inflammasome activation: 1 hour of LPS priming followed by 4 hours flagellin transfection. For with-in group analysis one-sided paired Wilcoxin test was used. For between group analysis Mann-Whitney test was used. Data presented as median with interquartile range, *p<0.05, **p<0.005, ***p<0.0001.