Figure 11.

The structure of the template-RTP RdRp complex. (A) The sequence of the RNA duplex with a 5′ U10 overhang as a template for extending primer and assembling the RdRp-RNA complex. (B) Cryo-EM Structure of the Remdesivir and RNA bound RdRp complex. Template and Primer are shown as yellow and cyan. (C) The conserved RdRp motifs (A to G) of the RNA-bound complex overlap with the apo structure, and color in gray, with a close view at the active site. Motifs (A to G) are shown in light orange, orange, blue, light blue, aquamarine, warm pink, and pale green, respectively. (D) A close view of the RdRp active site, showing the covalently bound RMP, magnesium ions, and pyrophosphate. Key residues and bases interacting with Remdesivir are shown. (E) Remdesivir-bound RdRp complex and protein-RNA interactions in the RNA. All structures are drawn by Pymol.