TABLE 4.

Differentially expressed nAChRs family subunit genes in 14-day-old adults with 1-, 10-, and 50-ppb imidacloprid treatments.

Bee stages	Gene ID	Imidacloprid treatments	Expression trend	Function	GO terms
14-day-old adults	GB43416	1, 10, 50 ppb	Up-regulated	Nicotinic acetylcholine receptor alpha6 subunit (nAChRa6)	GO:0016021	Integral component of membrane
					GO:0030054	Cell junction
					GO:0045211	Postsynaptic membrane
	GB40923	10, 50 ppb	Up-regulated	Nicotinic acetylcholine receptor alpha8 subunit (nAChRa8)	GO:0016021	Integral component of membrane
					GO:0030054	Cell junction
					GO:0045211	Postsynaptic membrane
	GB53427	1, 10, 50 ppb	Up-regulated	Nicotinic acetylcholine receptor alpha9 subunit (nAChRa9)	GO:0016021	Integral component of membrane
	GB53428	10, 50 ppb	Down-regulated	Nicotinic acetylcholine receptor beta2 subunit (nAChRb2)	GO:0016021	Integral component of membrane
	GB53055	50 ppb	Up-regulated	Nicotinic acetylcholine receptor beta1 subunit (nAChRb1)	GO:0016021	Integral component of membrane
					GO:0030054	Cell junction
					GO:0045211	Postsynaptic membrane