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. 2021 Jul 1;16(7):e0252805. doi: 10.1371/journal.pone.0252805

Fig 1. Screening monoclonal antibody clones against the amino terminus of CCL21.

Fig 1

A) Screening of CCL21 monoclonal antibody clones by western blotting for binding to the human CCL21 protein as a single band. Clone #8 (C8) results are shown for all assays in this figure. B) Cross reactivity of monoclonal anti-CCL21 antibody to human and mouse CCL21 protein. We then screened the clones for lack of binding to murine CCL21 by slot blot hybridization. C) Screening of a peptide from the CCR7-interacting amino terminal region of CCL21 could compete off the monoclonal from binding CCL21 using slot blot hybridization. D) Screening of monoclonal antibody clones for lack of binding to the related human chemokine CCL19. Thirty-three monoclonal antibodies passed all of these screens.