Appendix 2—table 2. The uptake of wogonin in four different cell lines.

The incubation concentration, time, condition and intracellular concentrations were summarized in the table. Inhibitor(s) were changed in different cell line. For OATP1B1 and OATP1B3 cell line, inhibitor was 50 μM rifampicin. For OATP 2B1 cell line, inhibitor was 50 μM telmisartan. For MDCK cell line, inhibitors were 50 μM rifampicin and 50 μM telmisartan. Student t test was applied to calculate the p values. No significant differences observed when wogonin was incubated with or without inhibitor.

Cell type	Incubation concentration(μM)	Incubation time(min)	Inhibitor	Intracellular concentration(nM)
MDCK MRP3 over-expressed cell line	5	120	Yes	150.33 ± 37.87
	5	120	No	163.67 ± 21.57
HEK-293 OATP1B1 over- expressed cell line	10	20	Yes	442.00 ± 309.56
	10	20	No	365.67 ± 168.67
HEK-293 OATP1B3 over- expressed cell line	10	60	Yes	333.33 ± 33.08
	10	60	No	381.33 ± 128.82
HEK-293 OATP2B1 over- expressed cell line	10	20	Yes	782.33 ± 115.42
	10	20	No	897.00 ± 409.17