Appendix 2—table 3. The uptake of wogonin and wogonin-7-G (wogonoside) in three different cell lines.

The incubation concentration, time, condition and intracellular concentrations were summarized iin the table. Inhibitor(s) were changed in different cell line. For OATP1B1 and OATP1B3 cell line, inhibitor was 50 μM rifampicin. For OATP 2B1 cell line, inhibitor was 50 μM telmisartan. Student t test was applied to calculate the p values. Significant differences were observed when wogonoside incubated with or without inhibitors in OATP 1B1 and 1B3 cell lines. No significant differences observed when wogonin was incubated with or without inhibitor.

Cell type	Incubation concentration (μM)	Incubation time (min)	Inhibitor	Wogonoside intracellular concentration (nM)	Wogonin intracellular concentration (nM)
HEK-293 OATP1B1 over- expressed cell line	10	20	Yes	0.01 ± 0.01	442.00 ± 309.56
	10	20	No	401.00 ± 29.60††	365.67 ± 168.67
HEK-293 OATP1B3 over- expressed cell line	10	60	Yes	48.33 ± 9.12	333.33 ± 33.08
	10	60	No	58.03 ± 16.60	381.33 ± 128.82
HEK-293 OATP2B1 over- expressed cell line	10	20	Yes	34.57 ± 21.26	782.33 ± 115.42
	10	20	No	82.87 ± 2.45*	897.00 ± 409.17

^*p< 0.05.

^†p<0.01.