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. 2021 Jun 21;10:e67290. doi: 10.7554/eLife.67290

Figure 3. Sp140–/– mice have elevated Ifnb transcripts during bacterial infection.

(A) Mice were infected with Mycobacterium tuberculosis and at 28 days post-infection, lungs were processed for total RNA, which was used for RT-qPCR. Combined results of two independent experiments. (B) Mice were infected with Legionella pneumophila and RT-qPCR (top panel) and CFU enumeration (bottom panel) was performed on lungs collected at indicated times. Combined results of two independent infections. All mice were bred in-house, Sp140–/– and Sp140+/– were littermates. (A, B) Mann-Whitney test. *p≤0.05; **p≤0.01; ***p≤0.005. CFU, colony-forming unit; RT-qPCR, real-time quantitative-polymerase chain reaction.

Figure 3.

Figure 3—figure supplement 1. BMMs from B6.Sst1S and Sp140–/– mice show increased cell death upon stimulation with polyI:C, which is dependent upon IFNAR signaling.

Figure 3—figure supplement 1.

LDH release from primary BMMs after 16–24 hr stimulation with 100 μg/mL polyI:C for (A) B6, Sp140–/–, B6.Sst1S, and B6.Sst1S Ifnar–/– mice. Results are technical duplicates, and representative of three independent experiments for B6 (three mice), B6.Sst1S (two mice), and Sp140–/– (two mice) samples and two independent experiments for B6.Sst1S Ifnar–/– sample (one mouse). (B) LDH release after polyI:C stimulation for primary BMMs from B6, Sp140–/–, and Sp140–/– Ifnar–/– mice. Results represent technical triplicates and are representative of two independent experiments for two mice per genotype. * p≤0.05; **p≤0.01; ***p≤0.005 as calculated with an unpaired t-test with Welch’s correction. BMM, bone marrow-derived macrophage; IFNAR, interferon alpha and beta receptor; LDH, lactate dehydrogenase.