Figure 4. Global gene expression analysis of Sp110^–/–, Sp140^–/–, and B6.Sst1^S lungs after Mycobacterium tuberculosis infection.

(A) PCA or (B) Euclidean distance analysis of all the samples. (C, D) Heatmaps of gene expression in log₂-fold change from M. tuberculosis-infected B6. Genes shown are those significantly different between Sp140^–/– and B6. (C) GSEA Hallmark inflammatory response; and (D) GO type I IFN response genes. (E) Volcano plot comparing Sp140^–/– to B6.Sst1^S expression. Dots in red are twofold differentially expressed with adjusted p-value ≤0.05. PCA, principal component analysis.

Figure 4—figure supplement 1. B6.Sst1^S and Sp140^–/– lungs exhibit elevated transcript levels of the interferon-stimulated gene Il1rn during Mycobacterium tuberculosis infection.

RT-qPCR for Il1rn (encodes IL-1Ra) extracted from lungs at 28 days post-infection with M. tuberculosis. Combined results of two independent experiments. Mann-Whitney test. *p≤0.05; **p≤0.01; ***p≤0.005. RT-qPCR, real-time quantitative polymerase chain reaction.

Figure 4—figure supplement 2. Expression of SP family members in Sp140^–/– and Sp110^–/– mouse lungs during Mycobacterium tuberculosis infection.

Log₂-fold change and adjusted p-value for SP family members (Sp100, Sp110, and Sp140) from RNA-seq of M. tuberculosis-infected lungs from Sp110^–/– and Sp140^–/– mice, compared to B6 and B6.Sst1^S.