Figure 5. Susceptibility of Sp140^–/– to Mycobacterium tuberculosis and Legionella pneumophila is dependent on type I IFN signaling.

(A, B) Mice were infected with M. tuberculosis and measured for (A) body weight, and (B) bacterial burdens at day 25. Statistics in (A) show comparison to B6; data are from 9 B6, 13 Sp140^–/–, and 13 Sp140^–/– Ifnar^–/– mice. Combined results of two experiments. (C, D) Bacteria burden in L. pneumophila-infected mice at 96 hr. Combined results of two experiments. All mice were bred in-house (A, B, D); all but B6 were bred in-house (C). Mann-Whitney test (A–D). *p≤0.05; **p≤0.01; ***p≤0.005.

Figure 5—figure supplement 1. Antibody blockade of IFNAR1 reduces bacterial burden in Sp140^–/– mice during Mycobacterium tuberculosis infection.

Mice were infected with M. tuberculosis and treated with either IFNAR1-blocking antibody or isotype control starting 7 days post-infection. At 25 days post-infection, lungs were harvested to enumerate CFU. Results of one experiment. All mice were bred in-house. Mann-Whitney test. *p≤0.05; **p≤0.01; ***p≤0.005. CFU, colony-forming unit.