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. 2021 Jun 23;48(2):155. doi: 10.3892/ijmm.2021.4988

Figure 4.

Figure 4

Comparison of the expression level of calbindin-D28k in the duodenum and kidney. (A) Duodenum tissues were evaluated using calbindin-D28k immunohistochemical staining. (B) The Calbindin-D28k stained areas in the duodenum were calculated using ImageJ software version 1.51j8. (C) Kidney tissues were evaluated using calbindin-D28k immunohistochemical staining. (D) The Calbindin-D28k stained areas in kidney were calculated using ImageJ software version 1.51j8. The brown areas stained with diaminobenzidine were selected to measure the density as a percentage of the total area. Data represent the mean ± SEM. #P<0.05, ##P<0.01 vs. the normal group. Nor, normal group; ACD, allergic contact dermatitis; GC, glucocorticoid; TA, topically applied; SC, subcutaneously injected.