Figure 6. Protective effects of silibinin on gastritis induced by Helicobacter pylori plus high salt diet in mouse stomach.

(A) To induce gastritis, mice were inoculated with H. pylori (1 × 10⁸ CFUs/100 µL) 5 times and given high salt diet (7.5%) for 4 weeks, which was followed by administration of silibinin for 4 weeks or 8 weeks. Mice tissues were stained with H&E (magnification: ×200). Scale bar, 200 µm. Arrows indicate the inflamed sites. (B) After 10 weeks, mouse stomach tissues were collected and lysed for Western blot analysis of COX-2 and inducible nitric oxide synthase expression. Immunoblots were subjected to statistical analysis. *Significantly different between the groups compared (P < 0.05).