Fig. 1.

Pedigrees of the six nuclear families included in this study. All patients underwent detailed clinical interview and T. cruzi serological tests. T. cruzi-seropositive individuals were considered as CCC patients when presenting major ECG abnormalities according to the Minnesota Code classification, modified by Ribeiro et al. [8]. Major ECG abnormalities are cited in online Table 1. The presence of any one of these major ECG changes was associated with a twofold increase in mortality [8]. T. cruzi-seropositive individuals presenting major ECG abnormalities, according to the Minnesota Code classification, modified by Ribeiro et al. [8] were considered as CCC patients. T. cruzi-seropositive patients without such ECG findings, with normal echocardiography, and no clinical signs were considered indeterminate forms of CD (ASY) cases