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. 2021 Jun 17;12:686332. doi: 10.3389/fpls.2021.686332

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Copyright © 2021 Zhang, Vail, Duddu, Parkin, Guo, Johnson and Shirtliffe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The growth pattern of flowering progress for a Brassica napus line (NAM-23) during the flowering stage at the Agriculture and Agri-Food Canada Research Farm (52° 10' 59.3” N, 106° 30' 53.7” W) near Saskatoon, SK, Canada in 2017. The x-axis is the imagery acquisition date (Julian date) in 2017. The y-axis is normalized difference yellowness index (NDYI)-based pixel number per plot. A solid curve line is the flowering progress trend of NAM-23. Seven points on the progress curve line represent NDYI-based pixel number per plot at seven imagery acquisition dates. Seven pictures under each point are corresponding false-color images after thresholding with flowers highlighted in yellow. The region of interest was highlighted in red.