TABLE 5.

Effects of low (LT), control (CT), and high (HT) temperature of incubation on noradrenaline (NA), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), DOPAC/DA, serotonin (5-HT), 5-hydroxyindole-3-acetic acid (5-HIAA), and 5-HTIAA/5-HT in the diencephalon of 3- and 14-days-old female and male chicken.

		LT	CT	HT	P
3 days-old
NA (pg/μg)	♀	3.1 ± 0.3	3.6 ± 0.5	3.0 ± 0.5	ns
	♂	3.3 ± 0.4	3.3 ± 0.3	2.9 ± 0.4	ns
DA (pg/μg)	♀	0.4 ± 0.05	0.6 ± 0.1	0.5 ± 0.09	ns
	♂	0.3 ± 0.01	0.6 ± 0.03	0.7 ± 0.3	ns
DOPAC (pg/μg)	♀	0.2 ± 0.02	0.2 ± 0.03	0.2 ± 0.03	ns
	♂	0.2 ± 0.02	0.2 ± 0.02	0.2 ± 0.04	ns
DOPAC/DA	♀	0.4 ± 0.02	0.4 ± 0.07	0.6 ± 0.08	ns
	♂	0.4 ± 0.04	0.3 ± 0.03	0.4 ± 0.05	ns
5-HT (pg/μg)	♀	3.7 ± 0.4	5.2 ± 1.2	3.6 ± 0.5	ns
	♂	4.3 ± 0.6	4.2 ± 0.2	4.0 ± 0.3	ns
5-HTIAA (pg/μg)	♀	1.1 ± 0.1	1.1 ± 0.2	1.3 ± 0.1	ns
	♂	1.3 ± 0.1	1.2 ± 0.1	1.2 ± 0.2	ns
5-HTIAA/5-HT	♀	0.3 ± 0.04	0.2 ± 0.05	0.4 ± 0.02	ns
	♂	0.3 ± 0.04	0.3 ± 0.05	0.3 ± 0.04	ns
14 days-old
NA (pg/μg)	♀	5.9 ± 0.7	6.2 ± 0.6	5.8 ± 0.9	ns
	♂	5.5 ± 0.3	5.4 ± 0.5	4.3 ± 1.0	ns
DA (pg/μg)	♀	1.1 ± 0.2	1.0 ± 0.09	0.9 ± 0.2	ns
	♂	1.2 ± 0.2	1.0 ± 0.05	0.8 ± 0.2	ns
DOPAC (pg/μg)	♀	0.2 ± 0.03	0.2 ± 0.03	0.2 ± 0.02	ns
	♂	0.3 ± 0.04	0.3 ± 0.02	0.3 ± 0.04	ns
DOPAC/DA	♀	0.3 ± 0.01	0.2 ± 0.03	0.2 ± 0.03	ns
	♂	0.2 ± 0.02	0.3 ± 0.01	0.3 ± 0.07	ns
5-HT (pg/μg)	♀	6.8 ± 0.8	7.3 ± 0.8	6.4 ± 0.8	ns
	♂	7.8 ± 0.8	7.6 ± 0.5	5.4 ± 1.3	ns
5-HTIAA (pg/μg)	♀	0.8 ± 0.1	0.9 ± 0.1	0.6 ± 0.03	ns
	♂	0.9 ± 0.09	0.8 ± 0.06	0.7 ± 0.1	ns
5-HTIAA/5-HT	♀	0.1 ± 0.01	0.1 ± 0.02	0.1 ± 0.01	ns
	♂	0.1 ± 0.01	0.1 ± 0.003	0.1 ± 0.02	ns

Significance level (P ≤ 0.05). ns, not significant (P > 0.05). [3d ♀ (n = 17); 3d ♂ (n = 15); 14d ♀ (n = 18); 14d ♂ (n = 17)].