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. 2021 Jul 1;11:13627. doi: 10.1038/s41598-021-93164-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The differences between the diabetic innervated constructs from the healthy. (A) Heat map representing all significant metabolites found between the healthy, T1DM and T2DM innervated constructs, as determined by one-way ANOVA on Metaboanalyst. (B) The top 4 pathways most significantly altered between the T1DM innervated constructs and the healthy, and the T2DM innervated constructs and the healthy. (C) The number of metabolites altered in the T1DM and T2DM innervated constructs when compared to the healthy. (D–F) graphs exhibiting (D) metabolites altered in both T1DM and T2DM innervated constructs, (E) those of the T1DM innervated constructs, and (F) those of the T2DM innervated constructs. n = 3–4, student’s t test, p: *≤ 0.05; **≤ 0.01; ***≤ 0.001; ****≤ 0.0001.