Table 2.
Nuclear, cytoplasmic, and membranous DDIT4 expression in colorectal cancer (CRC) tissues and their adjacent normal tissue samples (Intensity of staining, percentage of positive tumor cells, and H-score).
| Scoring system | Nuclear DDIT4 N (%) | Adjacent normal N (%) | P value | Cytoplasmic DDIT4 N (%) | Adjacent normal N (%) | P value | Membranous DDIT4 N (%) | Adjacent normal N (%) | P value |
|---|---|---|---|---|---|---|---|---|---|
| Intensity of staining | 0.004 | 0.023 | 0.013 | ||||||
| No staining (0) | 0 (0.0) | 1 (2.6) | 0 (0.0) | 0 (0.0) | 2 (1.0) | 0 (0.0) | |||
| Weak (+ 1) | 6 (3.0) | 1 (2.6) | 12 (6.1) | 7 (17.9) | 6 (3.0) | 2 (5.1) | |||
| Moderate (+ 2) | 86 (43.4) | 26 (66.7) | 134 (67.7) | 26 (66.7) | 101 (51.0) | 28 (71.8) | |||
| Strong (+ 3) | 106 (53.5) | 11 (28.1) | 52 (26.2) | 6 (15.4) | 89 (45.0) | 9 (23.1) | |||
| Percentage of positive tumor cells | 0.781 | 0.533 | 0.086 | ||||||
| < 25% | 1 (0.5) | 1 (2.6) | 0 (0.0) | 0 (0.0) | 4 (2.0) | 0 (0.0) | |||
| 25–50% | 4 (2.0) | 1 (2.6) | 6 (3.0) | 3 (7.7) | 7 (3.5) | 1 (2.6) | |||
| 51–75% | 53 (26.8) | 10 (25.6) | 73 (36.9) | 14 (35.9) | 69 (34.8) | 9 (23.1) | |||
| > 75% | 140 (70.7) | 27 (69.2) | 119 (60.1) | 22 (56.4) | 118 (59.6) | 29 (74.4) | |||
| H-score cut off | 0.003 | 0.142 | 0.211 | ||||||
| Low | 93 (47.0) | 22 (56.4) | 120 (60.6) | 25 (64.1) | 115 (58.1) | 31 (79.5) | |||
| High | 105 (53.0) | 17 (43.6) | 78 (39.4) | 14 (35.9) | 83 (41.9) | 8 (20.5) | |||
| Total | 198 | 39 | 198 | 39 | 198 | 39 |
P value is based on Mann–Whitney U test.
H-score histological score.