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. 2021 Jul 1;12:4068. doi: 10.1038/s41467-021-24342-y

Fig. 4. TMPRSS2 is androgen regulated in lung cell lines.

Fig. 4

A549, H1944 and BEAS-2B were incubated in hormone-depleted media for 72 h and treated with ±10 nM dihydrotestosterone (DHT) ± 10 μM enzalutamide (ENZA) for 24 h. RNA was harvested, reverse transcribed and qPCR performed to quantify a TMPRSS2 and b FKBP5 expression. Mean of at least three independent repeats (±1 SEM). Significance determined using one-way ANOVA with Dunnett’s multiple comparison test. TMPRSS2: A549 Vehicle Control (VC) v DHT p = 0.0190, DHT v ENZA p = 0.0228; H1944 VC v DHT p = 0.0410, DHT v ENZA p = 0.0497. FKBP5: A549 VC v DHT p = 0.0250, DHT v ENZA p = 0.0493, DHT v DHT + ENZA p = 0.0285; H1944 VC v DHT p = 5.7 × 10–6, DHT v ENZA p = 7.1 × 10–5, DHT v DHT + ENZA p = 1.7 × 10–5. c A549 were incubated in hormone-depleted media for 72 h and treated with ±10 nM DHT ± 10 μM ENZA for 24 h. Cells were lysed and proteins separated using SDS-PAGE. Immunoblotting was performed to visualise AR and TMPRSS2 expression levels and β−actin used as a loading control. Densitometry was performed for AR and TMPRSS2, values normalised to β−actin and made relative to VC. Representative immunoblots blots – A549 n = 3, H1944 n = 2. Source data are provided as a Source Data file.