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. 2021 Jul 1;11:13700. doi: 10.1038/s41598-021-93156-1

Figure 1.

Figure 1

Generation and validation of the experimental model. e-AMPK WT/KO mice were intraperitoneally administered Tamoxifen (500 µg/mice) for 5 consecutive days in order to induce ⍺1AMPK invalidation specifically in the endothelium. 3 weeks after the last Tamoxifen injection, mice were sacrificed and endothelial cells were immunoprecipitated from lung tissue with dynabeads. ⍺1AMPK gene expression was detected by TaqMan qPCR on both isolated endothelial cells and supernatants. (a) Validation of endothelial ⍺1AMPK deletion’s extent in e-AMPK KO. ⍺1AMPK expression detected by TaqMan qPCR in immunoprecipitated endothelial cells. (b) Validation of endothelial ⍺1AMPK deletion’s specificity. ⍺1AMPK expression detected by TaqMan qPCR on lysates of lung tissue depleted of endothelial cells. (c) Time course of canagliflozin plasma levels in mice treated by oral gavage, 100 mg/kg, during indicated time. The data are mean ± SEM, n = 3 to 5/group. The data were analyzed using one-way ANOVA test.