FIGURE 5.

Inhibition of Epstein–Barr virus (EBV) replication in Akata cells abolishes its enhancement effect on DENV-2 replication. (A) EBV + Akata cells were treated with dimethyl sulfoxide (DMSO), acyclovir (ACV), anti-human IgG antibody and DMSO, or anti-human IgG and ACV as described in section “Materials and Methods.” Relative EBV genome copy number was measured by qPCR. (B) EBV- Akata cells were infected with DENV-2 at a multiplicity of infection (MOI) of 10, DENV-2 titer in culture medium at 24 and 36 hpi was determined by plaque assay. (C) The four groups from panel A were infected with 10 MOI of DENV-2. At 24 and 36 hpi, DENV-2 yields from the infected EBV + Akata cells were titrated by plaque assay. p < 0.01 was marked as ** and p < 0.05 was marked as *.