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. 2021 Jul 1;11:13699. doi: 10.1038/s41598-021-93155-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IV iron treatment did not affect expression of iron metabolism genes significantly in normal or tumour tissues compared with oral iron treatment. (a) Real-time PCR fold change in SLC11A2 (DMT1) gene expression comparing oral versus intravenous iron groups. (b) Immunostaining of DMT1 in normal and Tumour tissues. (c) Analysis for DMT1 protein, dot plots with mean and standard deviation. (d) Real-time PCR fold change in IREB2 gene expression comparing oral versus intravenous iron groups. (e) IREB correlation with DMT.