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. 2021 Jul 1;4:815. doi: 10.1038/s42003-021-02275-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a 3D rendering of a large data subset derived from a cleared tumor dissected from an animal after IV injections of TRITC-albumin (green) and AF647-lectin (red). Scale bar, 500 µm. b, c 3D rendering of the regions marked in a in higher magnification. d, e TRITC-albumin extravasation segmented by thresholding after subtraction of the segmented vasculature in the tissue subsets in b and c, the colormap reflects the density of the pixels in the extravasation mask. f, g Traced vasculature from the subsets in b and c colored according to the vessel diameter, numbers for minimum and maximum vessel diameter are presented in µm. Scale bars, 100 µm. h Total volume of extravasation in the tissue subsets. i Vascular volume fraction in the tissue subsets. j Mean diameter of the vascular segments in tissue subsets. Data are presented as mean ± SD (n = 4804 and 5387 individual vessel segments). k Histogram representing the volume fraction occupied by vessels of a certain diameter from the total vascular volume fraction in the tissue subsets. l Mean length of the vascular segments in the tissue subsets. Data are presented as mean ± SD (n = 4804 and 5387 individual vessel segments). m Number of branching points per volume in the tissue subsets. n Mean tortuosity of the vasculature in the tissue subsets. Data are presented as mean ± SD, (n = 4804 and 5387 individual vessel segments).