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. 2021 Jul 1;11:13638. doi: 10.1038/s41598-021-92940-3

Figure 1.

Figure 1

Calu-3 cells respond to SARS-CoV-2 infection by upregulating type I and III IFNs, ISGs and cytokines. (A) The indicated cell lines were mock-infected or infected with SARS-CoV-2 (MOI = 0.1 or 0.5) for 24 h prior to RNA extraction and RT-qPCR for the indicated transcripts. Data are relative to GAPDH expression. (B) Calu-3 cells were stimulated with 100 U/ml IFN-A/D for 16 h. Cell lysates were analysed by western blot using the indicated antibodies. Individual membranes were probed for each protein. Membranes were subsequently re-probed for β-actin. See Supplementary Figure 2 for full blots. Data are from a single experiment. Data points in (A) are from technical duplicates and bars indicate the average.